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Fear Conditioning - Freeze Monitoring

Fear Conditioning System


  • Fusion Software Supports up to 16 Cages per PC
  • Utilizes the Stimulus Hub and the Stimulus Profile Generator for sensory output (Light,Sound and Shock ).
  • Sophisticated pre-pulse stimuli control - programmable intensity, frequency, start time, and duration for Light and Sound Stimuli.
  • Comprehensive data analysis gives precise information about the freezing behavior of each animal.
  • Up to 10 Locomotor Axes per Cage
  • 16 Photobeams per Locomotor Axis
  • Captures 100 Data points per second per Cage(all 10 Axes)
  • Stagger Start/Stop Switches Integrated into each Axis
  • Advanced Diagnostic Utilities
  • Records Complete Experimental Data
  • Advanced Playback of Experiments
  • Comprehensive Security Features
  • Modular and Extensible Fusion Software (XP/Vista Compatible)
  • USB Compatible/CE Compliant


Can Be Combined With


Fear Conditioning - Freeze Monitoring is a system designed to gauge animal responses to anxiety inducing stimuli. The system is capable of sending three different kinds of stimuli; light, sound and shock,. Moreover, because of the integration with the Fusion system, it is capable of tracking the animals’ movement as the stimuli are activated.

Hardware Requirements

  • Open Field activity monitor
  • Stimulus Hub with shock generator
  • Stimulus Lid with speaker and LED light
  • Test Cage with Shock Tray


The system can run tests on 16 animals simultaneously. Each test chamber includes a sound stimulus (10 to 20,000 Hz pure sine tone), light stimulus, multi-pole scrambled foot shocker, open field activity monitor and ventilated sound proof box(optional).Duration and Intensity of all 3 stimuli as well as the stimuli sequence can be programmed via the Stimulus Profile Generator.

Measuring Latency

Experiments containing stimulus events can be analyzed using the stimulus event file. This analysis contains, among other things, latency variables.
The Fusion software determines latencies to locomotor activity, ambulation, and rearing.
Latencies are measured from the start and end of each stimulus event. If no movement is detected before the end of the repetition, the latency will be blank.


The experiment is divided into one or more “Repetitions” which each consist of a light, sound, and shock event. In the fear conditioning paradigm, the shock event must follow the light and sound events (pre-pulse) in each repetition. Light and Sound can occur in any order. Some of the parameters included are:
light delay,intensity and duration ;
sound delay,intensity, duration and frequency ;
shock delay,intensity and duration ;
repetition count and more.
The Inter Repetition Period can be Fixed or Randomly variable.